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Medium optimization of carbon and nitrogen sources for the production of eucalyptene A and xyloketal A from Xylaria sp. 2508 using response surface methodology

Paper ID Volume ID Publish Year Pages File Format Full-Text
36162 45123 2006 6 PDF Available
Title
Medium optimization of carbon and nitrogen sources for the production of eucalyptene A and xyloketal A from Xylaria sp. 2508 using response surface methodology
Abstract

To optimize the production of novel ketal compounds and allenic ethers from a mangrove fungus Xylaria sp. 2508, optimization of the culture medium was carried out using a two-step approach. A quick identification of a suitable source of carbon and nitrogen was obtained by a simple screening experiment, which was followed by an application of response surface methodology (RSM) for further optimization design. A second order model was found to fit the eucalyptene A production. Response surface analysis revealed that the optimum values of the tested variables for the production of eucalyptene A were: glucose 2.86% (w/v), peptone 0.78% (w/v) and yeast extract 0.35% (w/v). A production of 4.89 × 10−7 g ml−1 for eucalyptene A, which was close to the predicted response, was observed in verification experiment. The production of xyloketal A was 2.20 × 10−7 g ml−1. In comparison to their production of basal medium, 1.53- and 1.32-fold increase had been obtained, respectively. The 3-D response surface curves implied that high yeast extract concentration repressed the growth of Xylaria sp.

Keywords
Medium optimization; Ketal compounds; Allenic ethers; Xylaria sp.; Response surface methodology
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Medium optimization of carbon and nitrogen sources for the production of eucalyptene A and xyloketal A from Xylaria sp. 2508 using response surface methodology
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 41, Issue 2, February 2006, Pages 293–298
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us