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Response surface optimization of antidipteran delta-endotoxin production by Bacillus thuringiensis subsp. israelensis HD 500

Paper ID Volume ID Publish Year Pages File Format Full-Text
36169 45123 2006 6 PDF Available
Title
Response surface optimization of antidipteran delta-endotoxin production by Bacillus thuringiensis subsp. israelensis HD 500
Abstract

Response surface optimization of Bacillus thuringiensis subsp. israelensis HD 500 fermentation for the production of its toxin proteins Cry4Ba and Cry11Aa was performed. Since the interaction of the medium components as well as cultivation conditions were expected to influence the production of the toxin proteins, an experimental chart was prepared by accepting the previously reported optimal values for the most important parameters as zero points: [Mn], 10−6 M; [K2HPO4], 50 mM; C:N ratio, 20:1 and incubation temperature; 30 °C. When the combinations of these variables at different levels were studied at 30 batch cultures and analysed for the optimum toxin protein concentrations: temperature, 28.3 °C; [Mn], 3.3 × 10−7 M; C:N ratio, 22.2 and [K2HPO4], 66.1 mM yielded the highest concentrations of both Cry4Ba and Cry11Aa toxin proteins.

Keywords
Bacillus thuringiensis; Delta-endotoxin; Cry4Ba; Cry11Aa; Fermentation; Response surface optimization
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Response surface optimization of antidipteran delta-endotoxin production by Bacillus thuringiensis subsp. israelensis HD 500
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 41, Issue 2, February 2006, Pages 350–355
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us