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Development of a biofilm technology for the production of 1,3-propanediol (1,3-PDO) from crude glycerol

Paper ID Volume ID Publish Year Pages File Format Full-Text
3619 178 2012 7 PDF Available
Title
Development of a biofilm technology for the production of 1,3-propanediol (1,3-PDO) from crude glycerol
Abstract

Glycerol is the main by-product of transesterification of fats in the biodiesel production. 1,3-Propanediol (1,3-PDO) is a valuable chemical that can be obtained from glycerol by microbial conversion. A number of Enterobacteriaceae species are able to produce 1,3-PDO from glycerol in stirred tank freely suspended cell bioreactors. Little is known about the use of crude glycerol in the production of 1,3-PDO and about the opportunity to intensify the process via strain immobilization in packed bed bioreactors.In this work, Citrobacter freundii, strain DSM 15979, and Pantoea agglomerans, strain DSM 30077, were tested for their ability to produce 1,3-PDO from crude glycerol in shaken flask batch conditions and in packed bed biofilm reactors operating under continuous conditions. Three different hydraulic retention times (HRT) were comparatively tested (8, 4 and 2 h) in order to understand its effects on 1,3-PDO production under immobilized cell conditions. The study revealed that HRT significantly influenced the process performances. The best productivities were observed when a HRT of 2 h was applied. However, both strains were found to be good candidates for 1,3-PDO production in biofilm reactors, even though P. agglomerans displayed quite higher productivities (3.6 g/(L h)) than the other strain.

► Immobilization via attachment on polyurethane foam and Vukopor®. ► 1,3-Propanediol productivities are higher when shorter hydraulic retention times were used. ► Immobilization of microbial cells provide resistance to extreme environmental conditions.

Keywords
1,3-Propanediol; Citrobacter freundii; Fermentation; Glycerol immobilized cells; Packed bed bioreactors; Pantoea agglomerans
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Development of a biofilm technology for the production of 1,3-propanediol (1,3-PDO) from crude glycerol
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 64, 15 May 2012, Pages 84–90
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us