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A novel α-glucosidase from Chaetomium thermophilum var. coprophilum that converts maltose into trehalose: Purification and partial characterisation of the enzyme

Paper ID Volume ID Publish Year Pages File Format Full-Text
36192 45124 2006 7 PDF Available
Title
A novel α-glucosidase from Chaetomium thermophilum var. coprophilum that converts maltose into trehalose: Purification and partial characterisation of the enzyme
Abstract

A constitutive extracellular α-glucosidase from Chaetomium thermophilum var. coprophilum was purified and characterised. The enzyme exhibited a carbohydrate content of 14% and an apparent molecular mass of 107 kDa, estimated by gel filtration. Analysis by SDS-PAGE revealed two polypeptide bands of 22 and 26 kDa, suggesting that the enzyme was constituted of two subunits of 22 kDa and two subunits of 26 kDa. Optima of pH and temperature were 7.0 and 60 °C, respectively. The enzyme hydrolysed maltose, amylose, starch, maltooligosaccharides and isomaltose, in order of preference. The enzyme exhibited transglycosylation activity producing trehalose and maltooligosaccharides from 5% maltose. At early stages of the transglycosylation reaction trehalose was the predominant product; at later stages maltooligosaccharides were also accumulated. Trehalose inhibited both glycosyl hydrolase and transglycosylase activities. To our knowledge this is the first report of a α-glucosidase from a filamentous fungus that in addition to glycosyl hydrolase activity produces trehalose by transglycosylation.

Keywords
Maltase; α-Glucosidase; Maltooligosyl trehalose synthase; Chaetomium thermophilum
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A novel α-glucosidase from Chaetomium thermophilum var. coprophilum that converts maltose into trehalose: Purification and partial characterisation of the enzyme
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 41, Issue 8, August 2006, Pages 1729–1735
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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Any Questions? feel free to contact us