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Application of statistical methodology to the optimization of fermentative medium for carotenoids production by Rhodobacter sphaeroides

Paper ID Volume ID Publish Year Pages File Format Full-Text
36198 45124 2006 6 PDF Available
Title
Application of statistical methodology to the optimization of fermentative medium for carotenoids production by Rhodobacter sphaeroides
Abstract

A statistical methodology, combining Plackett–Burman design (PBD) with uniform design (UD), was applied to optimize the concentrations of different inorganic salt components in liquid fermentative medium for carotenoids production by Rhodobacter sphaeroides. Among eleven inorganic salts investigated with PBD, MgSO4, Na2HPO4, FeSO4 and Na2CO3 were selected out based on their statistically significant (P < 0.05) and positive effects on carotenoids yield. UD was used for further optimization of those selected inorganic salts in order to increase carotenoids output. By step-wise analysis to the data from UD, a second-order polynomial model was established to identify the relationship between the carotenoids output and these four inorganic salts. The optimum combination of inorganic salts for carotenoids yield was evaluated with the non-linear optimization algorithm as follows: MgSO4 0.12 g/L, Na2HPO4 2.05 g/L, FeSO4 0.03 g/L, Na2CO3 2.22 g/L, and the other components of fermentative medium were kept the same as basal medium (citric acid 8.1 g/L, NH4Cl 3.5 g/L, corn syrup 1.8 g/L). A maximum carotenoids yield of 17.245 mg/L was demonstrated by confirmatory experiment to the optimum medium in 5-day fermentation.

Keywords
Photosynthetic bacteria; Rhodobacter sphaeroides; Carotenoids; Inorganic salts; Plackett–Burman design; Uniform design
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Application of statistical methodology to the optimization of fermentative medium for carotenoids production by Rhodobacter sphaeroides
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 41, Issue 8, August 2006, Pages 1773–1778
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us