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Capsular polysaccharide production by Neisseria meningitidis serogroup C: Optimization of process variables using response surface methodology

Paper ID Volume ID Publish Year Pages File Format Full-Text
36205 45124 2006 7 PDF Available
Title
Capsular polysaccharide production by Neisseria meningitidis serogroup C: Optimization of process variables using response surface methodology
Abstract

The production of capsular polysaccharide by Neisseria meningitidis serogroup C in Frantz medium cultivations was studied. Influence of process variables such as temperature, pH and agitation speed on the polysaccharide production were investigated by means of a three-factor face-centered central composite design. An optimal set of those process variables values were calculated using response surface methodology, and results showed that optimal values were: temperature equal to 37 °C, pH controlled at 7.0 and agitation speed maintained at 1300 rpm, which correspond to a oxygen global mass transfer coefficient (kLa) of 36 h−1. The capsular polysaccharide concentration achieved under these conditions was equal to 155 ± 4 mg/L.

Keywords
Neisseria meningitidis; Capsular polysaccharide; Face-centered central composite design; Response surface methodology; Process variables optimization
First Page Preview
Capsular polysaccharide production by Neisseria meningitidis serogroup C: Optimization of process variables using response surface methodology
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 41, Issue 8, August 2006, Pages 1822–1828
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering