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Characterization of concentration and purification parameters and operating conditions for the small-scale recovery of surfactin

Paper ID Volume ID Publish Year Pages File Format Full-Text
36222 45125 2005 6 PDF Available
Title
Characterization of concentration and purification parameters and operating conditions for the small-scale recovery of surfactin
Abstract

Batch recovery including concentration and purification of surfactin from the cell-free fermentation broth was carried out using an ultrafiltration membrane in a magnetically stirred cell system. The filtration characteristics of surfactin on passing through the polymeric membrane, such as permeate flux, rejection coefficient, concentration factor, solute yield or purification factor and recovery factor were evaluated. The effects of the trans-membrane pressure, initial surfactin concentration and the pH of the surfactin solution on the permeate flux were also determined. A maximum of 166-fold concentration of surfactin was rapidly achieved under the optimum trans-membrane pressure and solution pH. The feed surfactin concentration of about 0.5 g L−1 resulted in the formation of a gel polarized layer which was difficult to remove by magnetic stirring, thereby decreasing the permeate flux and the solute yield. The present investigations also qualitatively compare the purity of the final product obtained through solvent extraction and ultrafiltration. The purity of surfactin obtained by ultrafiltration was found to be 70%, as calculated from critical micelle concentration value (CMC) of 0.017 g L−1 against that of 0.013 g L−1 for surfactin (Sigma, 98% pure), used as the reference standard. Thus, an understanding of ultrafiltration characteristics and operating conditions from the analyses of the present study constitutes a very important milestone for process optimization and scale-up.

Keywords
Surfactin; Ultrafiltration characteristics; Operating conditions; CMC; % Purity
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Characterization of concentration and purification parameters and operating conditions for the small-scale recovery of surfactin
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 40, Issue 9, September 2005, Pages 2953–2958
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us