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Studies on the heterotrophic cultivation of transgenic Chlorella containing the rabbit defensin gene

Paper ID Volume ID Publish Year Pages File Format Full-Text
36237 45125 2005 6 PDF Available
Title
Studies on the heterotrophic cultivation of transgenic Chlorella containing the rabbit defensin gene
Abstract

The heterotrophic culture of transgenic Chlorella containing the rabbit defensin (NP-1) gene was studied in a 5 L stirred tank bioreactor. An initial batch culture experiment indicated substrate shortage in the later phase of the culture. A preliminary fed-batch culture with glucose and KNO3 feeding as well as pH, and DO control was conducted. The transgenic Chlorella cell density reached about 9.6 g/L, which was 92% higher than the batch culture. The NP-1 gene expression capability maintained basically the same level. To enhance cell growth, a second fed-batch culture with a more finely designed scheme of feeding and process control was conducted, in which both the timing and frequency of substrate feeding varied. As a result, the final cell accumulation achieved about 17.0 g/L, which was almost double the preliminary experiment while the NP-1 gene expression capability showed no significant changes.

Keywords
Transgenic Chlorella; Rabbit defensin; Bioreactor; Heterotrophic; Expression; Substrate feeding
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Studies on the heterotrophic cultivation of transgenic Chlorella containing the rabbit defensin gene
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 40, Issue 9, September 2005, Pages 3055–3060
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
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Price was $35.95
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