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Production of phoA promoter-controlled human epidermal growth factor in fed-batch cultures of Escherichia coli YK537 (pAET-8)

Paper ID Volume ID Publish Year Pages File Format Full-Text
36239 45125 2005 7 PDF Available
Title
Production of phoA promoter-controlled human epidermal growth factor in fed-batch cultures of Escherichia coli YK537 (pAET-8)
Abstract

Escherichia coli YK537 (pAET-8) was used to produce human epidermal growth factor (hEGF), which was expressed under the control of phoA promoter and secreted to the culture medium. In batch culture using a complex medium, phosphate was nearly depleted at 8 h, but subsequently accumulated and reached 3.8 mM. The high phosphate concentration resulted in a low hEGF expression level of 28 mg l−1. Hydrolysis of the organophosphorus in the complex medium by extracellular enzymes caused the accumulation of phosphate, and supply of glucose led to the consumption of the released phosphate. Feeding of glucose using a pH-stat strategy was effective in maintaining low phosphate and glucose levels, and avoided the accumulation of acetic acid. The production of hEGF reached 281 mg l−1. When the culture was fed with the essential amino acids leucine and proline, the production of recombinant hEGF reached 332 mg l−1.

Keywords
Human epidermal growth factor; phoA promoter; Phosphate; Fed-batch culture; pH-stat
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Production of phoA promoter-controlled human epidermal growth factor in fed-batch cultures of Escherichia coli YK537 (pAET-8)
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 40, Issue 9, September 2005, Pages 3068–3074
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us