fulltext.study @t Gmail

Production of cellulase/β-glucosidase by the mixed fungi culture Trichoderma reesei and Aspergillus phoenicis on dairy manure

Paper ID Volume ID Publish Year Pages File Format Full-Text
36242 45125 2005 8 PDF Available
Title
Production of cellulase/β-glucosidase by the mixed fungi culture Trichoderma reesei and Aspergillus phoenicis on dairy manure
Abstract

Trichoderma reesei was co-cultured with Aspergillus phoenicis using dairy manure as a substrate to produce cellulase with a high level of β-glucosidase. For pure cultures of T. reesei and A. phoenicis, the optimal media compositions were the same (10 g/L manure supplemented with 2 g/L KH2PO4, 2 mL/L tween-80 and 2 mg/L CoCl2), while the optimal temperature and pH were similar (25.5 °C and pH 5.76 for T. reesei; 28.2 °C and pH 5.14 for A. phoenicis). The mixed culture was therefore completed at 27 °C and pH 5.5, which is close to the optimal values for both fungi. The mixed culture resulted in a relatively high level of total cellulase and β-glucosidase. It was also found that a high manure solid concentration (>20 g/L) resulted in higher enzyme activities, probably due to an alleviation of the nutrients limitation at 10 g/L manure. The β-glucosidase activity and filter paper activity of the mixed fungi culture were 0.64 IU/mL and 1.54 FPU/mL, respectively, corresponding to a ratio of 0.41, which is an ideal ratio for hydrolyzing manure cellulose. To test the effectiveness of the enzymes produced by mixed culture, a crude enzyme broth was used for hydrolyzing the manure cellulose. The glucose concentration produced was significantly (p < 0.01) higher than the glucose obtained when using the commercial enzyme and the enzyme broth of the pure culture T. reesei. The results indicate that the mixed culture of T. reesei and A. phoenicis is an effective approach to produce a cellulolytic enzyme system for hydrolyzing manure cellulose.

Keywords
Cellulase; β-Glucosidase; Animal manure; Trichoderma reesei; Aspergillus phoenicis; Mixed culture
First Page Preview
Production of cellulase/β-glucosidase by the mixed fungi culture Trichoderma reesei and Aspergillus phoenicis on dairy manure
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 40, Issue 9, September 2005, Pages 3087–3094
Authors
, , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us