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Production of cell-bound phytase by Pichia anomala in an economical cane molasses medium: Optimization using statistical tools

Paper ID Volume ID Publish Year Pages File Format Full-Text
36243 45125 2005 8 PDF Available
Title
Production of cell-bound phytase by Pichia anomala in an economical cane molasses medium: Optimization using statistical tools
Abstract

Statistical experimental designs were applied for the optimization of cell-bound phytase production by Pichia anomala in a cost-effective cane molasses medium. Using Plackett–Burman design, cane molasses, urea and inoculum density were identified as significant factors and these factors were subsequently optimized using a central composite design. The optimum variables that supported maximum enzyme titre were 8% cane molasses, 0.4% urea and 2% inoculum density. The validity of the optimized variables was verified in shake-flasks as well as in a laboratory fermenter. An overall 5- and 1.6-fold enhancement in phytase titres (324–1689 U/g DYB) and biomass production (6.4–10 g/l) were attained due to optimization, respectively.

Keywords
Phytase; Pichia anomala; Plackett–Burman design; Response surface methodology
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Production of cell-bound phytase by Pichia anomala in an economical cane molasses medium: Optimization using statistical tools
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 40, Issue 9, September 2005, Pages 3095–3102
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us