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Effect of media and culture conditions on growth and hydrocarbon production by Botryococcus braunii

Paper ID Volume ID Publish Year Pages File Format Full-Text
36247 45125 2005 7 PDF Available
Title
Effect of media and culture conditions on growth and hydrocarbon production by Botryococcus braunii
Abstract

The influence of the media constituents potassium nitrate, magnesium sulphate, dihydrogen potassium phosphate and ferric citrate on growth and hydrocarbon production in Botryococcus braunii (SAG 30.81) was investigated using response surface methodology (RSM). Among the individual variables, potassium nitrate and ferric citrate exhibited marked effects on the response functions (yield of biomass and hydrocarbon production). A high correlation coefficient (r ≥ 0.93, p ≤ 0.01) indicated the suitability of second-order polynomials to predict the response function. The optimum concentration of dihydrogen potassium phosphate, potassium nitrate, magnesium sulphate and ferric citrate were 0.16, 0.05, 0.10 and 0.025 g L−1, respectively, in the medium where hydrocarbon production was about 50% on dry weight basis. The optimum concentrations of dihydrogen potassium phosphate, potassium nitrate, magnesium sulphate and ferric citrate were found to be 0.0195, 0.05, 0.02 and 0.0185 g L−1, respectively, in the medium for 0.65 g L−1 biomass and 50.6% (w/w) hydrocarbon production. B. braunii (SAG 30.81) was identified as A race by mass spectra of hydrocarbons it produced.

Keywords
Hydrocarbon production; Yield of biomass; Response surface methodology; Botryococcus braunii; Optimisation
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Effect of media and culture conditions on growth and hydrocarbon production by Botryococcus braunii
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 40, Issue 9, September 2005, Pages 3125–3131
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
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