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Efficient production of recombinant proteins in Escherichia coli using an improved l-arabinose-inducible T7 expression system

Paper ID Volume ID Publish Year Pages File Format Full-Text
36249 45125 2005 6 PDF Available
Title
Efficient production of recombinant proteins in Escherichia coli using an improved l-arabinose-inducible T7 expression system
Abstract

An l-arabinose-inducible T7 expression system was shown to be highly stringent in the regulation of cloned gene expression. However, this stringency was medium-dependent and missing in complex media. To ensure consistent expression efficiency, the system was improved by constructing T7lac promoter-containing plasmids subject to the control of thermo-labile lacI. As a result, LacZ was produced to reach a 128-fold increase over the uninduced level in rich media. Furthermore, a large production of carbamoylase using the developed system was successfully achieved in a lab-scale fermenter. The production of soluble protein was 10% of total cell protein content and the cell yield 30 g dry cell weight per litre. Taken together, these illustrate the great promise of this system for industrial use.

Keywords
T7 system; l-Arabinose promoter; Expression vectors; Protein overproductions
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Efficient production of recombinant proteins in Escherichia coli using an improved l-arabinose-inducible T7 expression system
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 40, Issue 9, September 2005, Pages 3137–3142
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us