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Tyrosinase immobilised on polyamide tubular membrane for the l-DOPA production: Total recycle and continuous reactor study

Paper ID Volume ID Publish Year Pages File Format Full-Text
3626 179 2012 6 PDF Available
Title
Tyrosinase immobilised on polyamide tubular membrane for the l-DOPA production: Total recycle and continuous reactor study
Abstract

The production of l-3,4-dihydroxyphenylalanine (l-DOPA) from l-tyrosine catalysed from mushroom tyrosinase immobilised in a continuous membrane reactor was studied and compared with free enzyme used in a stirred tank reactor. The enzyme was immobilised by cross-flow filtration in asymmetric tubular membranes made of polyamide having nominal molecular weight cut off of 20 kDa.Results showed that the immobilization procedure did not alter the catalytic properties of the enzyme. The immobilised tyrosinase showed high stability over 30 h. In a continuous bioreactor the specific activity of tyrosinase is higher than that obtained with the free enzyme and those reported in the literature. The continuous operation minimizes the oxidation of the l-DOPA and for this reason the productivity (22.54 mg L−1 h−1) is 95% higher than that obtained with the total recycle reactor.Results obtained in the present study are promising for the development of the bioreactor at larger scale.

► We study the production of l-DOPA using a biocatalytic zeolite membrane. ► We study the role of the zeolite membrane as free radical scavenger. ► Results: Biocatalytic zeolite membrane enhances the performance of the tyrosinase. ► Results: Possibility to regenerate the zeolite membrane by thermal treatment. ► Results: The specific activity of the immobilised enzyme was higher than free form.

Keywords
Immobilised enzyme; Tyrosinase; Polyamide membranes; Continuous membrane bioreactor; l-DOPA production; Specific activity and productivity
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Tyrosinase immobilised on polyamide tubular membrane for the l-DOPA production: Total recycle and continuous reactor study
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 66, 15 July 2012, Pages 14–19
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us