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Production and modification of bioactive biosurfactants

Paper ID Volume ID Publish Year Pages File Format Full-Text
36261 45126 2006 8 PDF Available
Title
Production and modification of bioactive biosurfactants
Abstract

The soil bacterium Tsukamurella spec. DSM 44370 produces a mixture of oligosaccharide lipids when cultivated on sunflower oil. In contrast cultivation with calendula oil as carbon source afforded a different product composition with overproduction of 2,3-di-O-acyl-β-d-glucopyranosyl-(1–2)-β-d-galactopyranosyl-(1–6)-4,6-di-O-acyl-α-d-glucopyranosyl-(1–1)-α-d-glucopyranose (GL 3) that amounted to 60% of the whole product. GL 3 and its parent tetrahexose backbone G 3 were then modified enzymatically with the lipase Novozyme 435 from Candida antarctica by addition of one and two oleic acid molecules to GL 3 and four molecules to G 3. The new glycolipids were shown to exhibit interesting surface activities compared to commercially available products, decreasing the surface tension of water to 23 mN/m. In addition these products showed novel biological activities through the inhibition of the activation of Epstein-Barr virus early antigen.

Keywords
Tsukamurella spec.; Di- and oligosaccharides; Calendula oil; Bioreactor cultivation; Lipase; β-Galactosidase
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Production and modification of bioactive biosurfactants
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 41, Issue 10, October 2006, Pages 2138–2145
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
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Price was $35.95
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