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Overexpression of genes of the dha regulon and its effects on cell growth, glycerol fermentation to 1,3-propanediol and plasmid stability in Klebsiella pneumoniae

Paper ID Volume ID Publish Year Pages File Format Full-Text
36264 45126 2006 10 PDF Available
Title
Overexpression of genes of the dha regulon and its effects on cell growth, glycerol fermentation to 1,3-propanediol and plasmid stability in Klebsiella pneumoniae
Abstract

Glycerol dehydratase that catalyses the conversion of glycerol to 3-hydroxypropionaldehyde was previously supposed to be a limiting step in the 1,3-propanediol production from glycerol. In this study, glycerol dehydratase was over-expressed separately or coordinately with 1,3-propanediol oxidoreductase in Klebsiella pneumoniaeDSM2026 to investigate its effects on the glycerol fermentation. The overexpression of glycerol dehydratase surprisingly led to a serious impairment of growth of K. pneumoniae in both continuous and batch cultures. Instability of the plasmids bearing the genes encoding glycerol dehydratase and/or 1,3-propanediol oxidoreductase was observed in recombinant cells, especially in anaerobic cultures using glycerol as sole carbon source. It is postulated that an imbalanced conversion of glycerol to the intermediate 3-hydroxypropionaldehyde and its further conversion to 1,3-propanediol and the associated accumulation and toxicity of 3-hydroxypropionaldehyde are responsible for the phenomena observed. Furthermore, the putative regulatory gene dhaR of the dha regulon was also overexpressed in K. pneumoniae. The increased expression of 1,3-propanediol oxidoreductase confirmed the role of DhaR as a positive regulator of the dhaT gene.

Keywords
Glycerol metabolism; Glycerol dehydratase; 1,3-Propanediol oxidoreductase; 3-Hydroxypropionaldehyde; DhaR; Klebsiella pneumoniae
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Overexpression of genes of the dha regulon and its effects on cell growth, glycerol fermentation to 1,3-propanediol and plasmid stability in Klebsiella pneumoniae
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 41, Issue 10, October 2006, Pages 2160–2169
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us