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Optimization of protease production by Microbacterium sp. in feather meal using response surface methodology

Paper ID Volume ID Publish Year Pages File Format Full-Text
36280 45127 2006 7 PDF Available
Title
Optimization of protease production by Microbacterium sp. in feather meal using response surface methodology
Abstract

A 23 factorial design was performed with the aim of optimizing protease production by a strain of Microbacterium sp. isolated from feathers in decomposition by response surface methodology. Protease production was first tested on different nitrogen source (casein, peptone, yeast extract, gelatin, soybean protein, feather meal and cheese whey). Feather meal was the selected substrate to test the effect of three variables on protease production (temperature, initial pH and feather meal concentration) by RSM. The point was chosen with these conditions: temperature 37 °C, initial pH 7.0 and feather meal concentration 12.5 g l−1. Statistical analysis of results showed that, in the range studied, only pH did not have a significant effect on protease production whereas interaction between pH and feather meal concentration was significant. The optimum conditions were 25 °C, initial pH 7.0 and 12.5 g l−1 of feather meal. Under these conditions, the model predicted a protease activity of 202.7 U ml−1.

Keywords
Actinomycetes; Microbacterium; Protease; Experimental design; Optimization
First Page Preview
Optimization of protease production by Microbacterium sp. in feather meal using response surface methodology
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 41, Issue 1, January 2006, Pages 67–73
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering