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Effect of substrate concentration on hydrogen production and 16S rDNA-based analysis of the microbial community in a continuous fermenter

Paper ID Volume ID Publish Year Pages File Format Full-Text
36297 45127 2006 9 PDF Available
Title
Effect of substrate concentration on hydrogen production and 16S rDNA-based analysis of the microbial community in a continuous fermenter
Abstract

The effect of substrate concentration on hydrogen production was investigated using a continuous-flow stirred-tank reactor (CSTR). Sucrose was used as a model substrate. The CSTR was started at a sucrose concentration of 30 g COD/L and exhibited stable H2 production for 271 days at inlet sucrose concentrations of 10–60 g COD/L. Hydrogen production depended on the substrate concentration such that the highest values of 1.09 mol H2/mol hexoseadded, 1.22 mol H2/mol hexoseconsumed, 7.65 L H2/L/d, and 3.80 L H2/g VSS/d were recorded at a sucrose concentration of 30 g COD/L. All bacterial species detected by polymerase chain reaction-denaturing gradient gel electrophoresis analysis were H2-producing Clostridium spp. At inlet sucrose concentrations below 20 g COD/L, the H2 yield per hexoseconsumed decreased along with a significant decrease in the n-butyrate/acetate ratio. At the same range of sucrose concentrations, Clostridium scatologenes (an H2-consuming acetogen) was found in the sludge. At inlet sucrose concentrations over 35 g COD/L, substrate overload occurred and caused a decrease in the carbohydrate degradation efficiency and H2 yield per hexoseadded.

Keywords
n-Butyrate/acetate ratio; Clostridium; Continuous-flow stirred-tank reactor; Hydrogen production; Polymerase chain reaction-denaturing gradient gel electrophoresis; Substrate concentration
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Effect of substrate concentration on hydrogen production and 16S rDNA-based analysis of the microbial community in a continuous fermenter
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 41, Issue 1, January 2006, Pages 199–207
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
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Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
Online Support
Any Questions? feel free to contact us