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Aqueous two-phase partitioning of xylanase produced by solid-state cultivation of Polyporus squamosus

Paper ID Volume ID Publish Year Pages File Format Full-Text
36302 45127 2006 4 PDF Available
Title
Aqueous two-phase partitioning of xylanase produced by solid-state cultivation of Polyporus squamosus
Abstract

The partitioning of xylanase, produced by solid-state cultivation of Polyporus squamosus, in aqueous two-phase system polyethylene glycol/ammonium sulphate was studied. In system 10% (w/w) polyethylene glycol 1500/20% (w/w) ammonium sulphate/70% (w/w) crude enzyme at pH 5.1 favourable partition coefficient and the top phase yield were obtained, 85.6% and 97.37%, respectively, followed by purification factor 4.8. This result suggests the possible integration of cultivation and primary downstream processing step by partitioning of xylanase in aqueous two-phase system.

Keywords
Aqueous two-phase system; Partitioning; Xylanase; Polyporus squamosus; Solid-state cultivation; Downstream processing
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 41, Issue 1, January 2006, Pages 232–235
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
Online Support
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