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Integrated biooxidation and acid dehydration process for monohydroxylation of aromatics

Paper ID Volume ID Publish Year Pages File Format Full-Text
36313 45128 2007 6 PDF Available
Title
Integrated biooxidation and acid dehydration process for monohydroxylation of aromatics
Abstract

We examined the performance of an integrated biooxidation and acid dehydration process for aromatic monohydroxylation using the production of o-cresol from toluene as the model conversion. A toluene cis-glycol dehydrogenase gene (todD)-disrupted mutant of Pseudomonas putida T-57 was employed as the whole cell biocatalyst for oxidizing toluene to toluene cis-glycol (TCG). After bioconversion of toluene to TCG, o-cresol was produced by adding HCl to the culture medium, since it was found that TCG became unstable at a low pH and underwent spontaneous dehydration. When the todD-disrupted mutant cells were grown in a two-liquid-phase culture system with oleyl alcohol as the organic solvent, this integrated process produced 40 g l−1 of o-cresol in the organic solvent phase at 30 h. The total concentration of o-cresol in the two-liquid-phase culture reached 6.6 g l−1 at 30 h, which was approximately four-fold greater than that in the single-liquid-phase culture.

Keywords
Biocatalysis; o-Cresol; Monohydroxylation; Pseudomonas putida; Toluene dioxygenase; Two-liquid-phase culture
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Integrated biooxidation and acid dehydration process for monohydroxylation of aromatics
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 42, Issue 1, January 2007, Pages 46–51
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us