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Characterization of alkaline protease from Vibrio fluvialis strain VM10 isolated from a mangrove sediment sample and its application as a laundry detergent additive

Paper ID Volume ID Publish Year Pages File Format Full-Text
36328 45129 2006 5 PDF Available
Title
Characterization of alkaline protease from Vibrio fluvialis strain VM10 isolated from a mangrove sediment sample and its application as a laundry detergent additive
Abstract

An extracellular alkaline protease producing strain was isolated from the sediment sample collected from a mangrove station in Cochin estuary and was identified as Vibrio fluvialis. The protease produced was purified to apparent homogeneity by ammonium sulphate precipitation and was determined to be a 33.5-kDa protease as shown by SDS-PAGE and its activity detected by casein zymography. This protease had optimum activity at pH 8 and temperature 55 °C. This was stable at alkaline pH range and up to a temperature of 50 °C, after incubation for 1 h. Hg2+ and Cu2+ showed complete inhibition of the enzyme activity, while Co2+, Ca2+ and Fe3+ were enhancing the activity. This protease was inhibited by the serine protease inhibitor PMSF and the metal chelator EDTA but not by the classic metalloprotease inhibitor 1,10 phenanthroline. The protease was found to be stable in presence of compounds like H2O2, SDS, and Triton-X-100 and also in some commonly used commercial detergents tested. The extracellular production of the enzyme, the pH and thermal stability, and the stability in presence of oxidants, surfactants and commercial detergents, suggest its possible use as a laundry additive.

Keywords
Alkaline protease; Vibrio fluvialis; Detergent; Bleach stability; Thermo stability; Characterization
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Characterization of alkaline protease from Vibrio fluvialis strain VM10 isolated from a mangrove sediment sample and its application as a laundry detergent additive
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 41, Issue 6, June 2006, Pages 1239–1243
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us