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Enzymatic saccharification of hydrogen peroxide-treated solids from hydrothermal processing of rice husks

Paper ID Volume ID Publish Year Pages File Format Full-Text
36329 45129 2006 9 PDF Available
Title
Enzymatic saccharification of hydrogen peroxide-treated solids from hydrothermal processing of rice husks
Abstract

Rice husks were subjected to hydrothermal processing in aqueous medium to cause the degradation of xylan to xylooligosaccharides. The resulting solid phase, with enhanced contents of cellulose and lignin, was assayed as a substrate for enzymatic hydrolysis either directly or after treatments with hydrogen peroxide in alkaline media under a variety of operational conditions. Solid yield, solid composition and susceptibility to enzymatic hydrolysis were assessed by empirical modelling. The susceptibility of the solids to enzymatic hydrolysis was greatly improved by peroxide treatments. Under selected conditions (oxidizing treatments with 50 g hydrogen peroxide/100 g solids at 80 °C for 4 h using a liquor to solid ratio of 15 g/g) lignin was removed in high proportion, and cellulose was almost quantitatively recovered in solid phase. In the subsequent enzymatic hydrolysis (using an enzyme/substrate ratio of 25 FPU/g at liquor to solid ratio of 15 g/g for 48 h), 67.9% of cellulose was converted into glucose, leading to a medium containing 43.5 g glucose/L. Higher cellulose conversions were achieved when the liquor to solid ratio employed in the enzymatic hydrolysis was increased, but then the final glucose concentrations decreased owing to the higher amount of liquid.

Keywords
Alkaline hydrogen peroxide; Enzymatic hydrolysis; Glucose; Hydrothermal treatments; Rice husks
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Enzymatic saccharification of hydrogen peroxide-treated solids from hydrothermal processing of rice husks
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 41, Issue 6, June 2006, Pages 1244–1252
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
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Price was $35.95
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