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Production, purification and characterization of nuclease p1 from Penicillium citrinum

Paper ID Volume ID Publish Year Pages File Format Full-Text
36334 45129 2006 6 PDF Available
Title
Production, purification and characterization of nuclease p1 from Penicillium citrinum
Abstract

Nuclease p1 produced by Penicillium citrinum was purified and characterized. Fermentation was carried out for 49 h for optimum production. The enzyme was purified to homogeneity according to SDS-PAGE by thermal deactivation, ultrafiltration, (NH4)2SO4 precipitation, phenyl sepharose chromatography, ion-exchange chromatography and gel filtration. This protocol improved 93-fold purification and 5.4% recovery of enzyme activity. The molecular mass of nuclease p1 from Penicillium citrinum was estimated to be in the range from 43 to 44 kDa. The enzyme showed an obligate requirement for Zn2+ for activity. K+ had a significant effect on enzyme activity. Mg2+ and Ca2+ had a slight positive effect on enzyme activity. While Cu2+, Co2+, Al3+, SDS and Triton X-100 were strong inhibitors of nuclease p1. The enzyme was completely stable in the pH range from 5 to 6.5 and had a pH optimum of 5.4. The optimum temperature of nuclease p1 was 69 °C and enzyme was stable up to 70 °C. Km and Vmax for the purified enzyme were 24.28 mg/ml and 0.36 mg/ml min, respectively, with RNA as substrate.

Keywords
Nuclease p1; Production; Purification; Characterization; Penicillium citrinum; 5′-Phosphodiesterase
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Production, purification and characterization of nuclease p1 from Penicillium citrinum
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 41, Issue 6, June 2006, Pages 1276–1281
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
Online Support
Any Questions? feel free to contact us