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Antioxidant and free radical-scavenging activities of wheat germ protein hydrolysates (WGPH) prepared with alcalase

Paper ID Volume ID Publish Year Pages File Format Full-Text
36337 45129 2006 7 PDF Available
Title
Antioxidant and free radical-scavenging activities of wheat germ protein hydrolysates (WGPH) prepared with alcalase
Abstract

Wheat germ protein hydrolysates (WGPH) were obtained by enzymatic hydrolysis of defatted wheat germ protein isolates using Alcalase 2.4L FG. The degree of hydrolysis (DH) of WGPH was determined to be about 25% using pH-stat method. The molecular mass distribution of WGPH was lower than 1500 Da. The antioxidant and free radical-scavenging activities of WGPH were investigated by employing several in vitro assay systems, including the linoleic acid emulsion model system, 1,1-diphenyl-2-picrylhydrazyl (DPPH)/superoxide/hydroxyl radical-scavenging, reducing power, and ferrous ion-chelating activity. The antioxidant activity of WGPH was close to that of α-tocopherol in a linoleic acid emulsion system. WGPH showed scavenging activity against free radicals such as DPPH, superoxide, and hydroxyl radicals. The radical-scavenging effect was in a dose-dependent manner, and the EC50 values for DPPH, superoxide, and hydroxyl radicals were found to be 1.30, 0.40 and 0.12 mg/mL, respectively. Moreover, WGPH also exhibited notable reducing power and strong chelating effect on Fe2+. The data obtained by the in vitro systems obviously established the antioxidant potency of WGPH.

Keywords
Wheat germ protein hydrolysates; Antioxidant activity; Free radical-scavenging activity; DPPH; Alcalase
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Antioxidant and free radical-scavenging activities of wheat germ protein hydrolysates (WGPH) prepared with alcalase
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 41, Issue 6, June 2006, Pages 1296–1302
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us