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Fermentative conversion of sucrose and pineapple waste into hydrogen gas in phosphate-buffered culture seeded with municipal sewage sludge

Paper ID Volume ID Publish Year Pages File Format Full-Text
36346 45129 2006 6 PDF Available
Title
Fermentative conversion of sucrose and pineapple waste into hydrogen gas in phosphate-buffered culture seeded with municipal sewage sludge
Abstract

Sucrose and pineapple waste (PAW) were used as the carbon substrate to produce H2 using municipal sewage sludge as the H2 producer. The effects of pH, substrate concentration and type of buffer on H2 production were studied. Regardless of the type of carbon substrate, phosphate-buffered medium exhibited much higher H2 production efficiency and lower CO2/H2 ratio than those obtained in medium containing bicarbonate buffer. For H2 production from sucrose-based medium, the best pH and carbon substrate (sucrose) concentration was 7.5 and 20 g COD/l, respectively, giving a maximum H2 production rate (vH2,max)(vH2,max) and H2 yield (YH2)(YH2) of 745 ml/h/l and 2.46 mol H2/mol sucrose (or 5.27 mol H2/g COD), respectively. When using PAW as the substrate, the H2 production performance was similar with or without additional nitrogen source, indicating that the PAW substrate contained sufficient nitrogen source for cell growth and H2 production. The vH2,maxvH2,max and YH2YH2 obtained from phosphate-buffered PAW medium (Medium B) was 383 ml/h/l and 5.92 mol H2/g COD, respectively, which is 95 and 64% higher than the carbonate-buffered medium (Medium D). Although attaining lower H2 production rate than sucrose medium, the PAW-based medium gave comparable H2 yield, suggesting the feasibility of using the renewable resource (PAW waste) for fermentative H2 production. Increasing the culture volume (using PAW medium) from 100 ml to 2.5 L (a 25-fold increase) caused a 20–40% decrease in the H2 production rate and H2 yield, probably due to poor mixing efficiency in the scale-up experiments.

Keywords
Dark H2 fermentation; Phosphate buffer; Sewage sludge; Sucrose; Pineapple waste
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Fermentative conversion of sucrose and pineapple waste into hydrogen gas in phosphate-buffered culture seeded with municipal sewage sludge
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 41, Issue 6, June 2006, Pages 1353–1358
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us