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Rhodococcus rhodochrous PA-34: A potential biocatalyst for acrylamide synthesis

Paper ID Volume ID Publish Year Pages File Format Full-Text
36347 45129 2006 5 PDF Available
Title
Rhodococcus rhodochrous PA-34: A potential biocatalyst for acrylamide synthesis
Abstract

Rhodococcus rhodochrous PA-34 exhibited 55 U mg−1 dcw nitrile hydratase (NHase) activity for the transformation of acrylonitrile to acrylamide. The culture conditions of R. rhodochrous PA-34 for the production of NHase and reaction conditions for this NHase mediated conversion of acrylonitrile to acrylamide were optimised. The maximum production of NHase (2.5 U mL−1 culture) was carried out by culturing R. rhodochrous PA-34 in the medium containing (L−1) 10.0 g glycerol, 5.0 g peptone, 3.0 g yeast extract, 20 mg CoCl2, pH 7.0, 2 mL propionitrile at 30 °C for 36 h in an incubator shaker (160 rpm). The maximum conversion of acrylonitrile to acrylamide was observed in 100 mM potassium phosphate buffer (pH 7.0), at 10 °C, acrylonitrile (8%, w/v, i.e. 1500 mM) using resting cells of this organism corresponding to 1.6 mg dry cell weight (dcw) mL−1 reaction mixture (specific activity 55 U mg−1 dcw) in 1 h. The resting cells of R. rhodochrous PA-34 retained 76% NHase activity even in the presence of 20% (w/v) acrylonitrile. The conversion of acrylonitrile to acrylamide was carried out at 1 L scale and 600 g (8442 mM) of acrylamide was produced in 12 h at 10 °C.

Keywords
Rhodococcus rhodochrous PA-34; Nitrile hydratase (NHase); Acrylonitrile; Acrylamide
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Rhodococcus rhodochrous PA-34: A potential biocatalyst for acrylamide synthesis
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 41, Issue 6, June 2006, Pages 1359–1363
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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Any Questions? feel free to contact us