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Potentiometric bienzymatic biosensor based on PVC membrane containing palmitic acid for determination of creatine

Paper ID Volume ID Publish Year Pages File Format Full-Text
36349 45129 2006 7 PDF Available
Title
Potentiometric bienzymatic biosensor based on PVC membrane containing palmitic acid for determination of creatine
Abstract

Creatine bienzymatic potentiometric biosensors were prepared by immobilizing urease and creatinase with four different procedures on poly(vinylchloride) (PVC) ammonium membrane electrode containing palmitic acid prepared by using nonactine as an ammonium-ionophore. The analytical characteristics were investigated and were compared with those of the biosensor prepared by using carboxylated PVC. The effects of pH, buffer concentration, temperature, enzyme concentration and stirring rate, and enzyme immobilization procedures on the response to creatine of the biosensor were investigated. The linear working range and sensitivity of the biosensor were also determined. The creatine biosensor prepared by using the PVC membranes containing palmitic acid showed more effective performance than those of the carboxylated PVC based biosensors. Additionally, creatine assay in serum was successfully carried out by using the standard addition method without any pretreatment. The results were good agreement with those obtained from Jaffe methods at 95% confidence level.

Keywords
Creatine biosensor; Potentiometric; Creatine determination; Palmitic acid
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Potentiometric bienzymatic biosensor based on PVC membrane containing palmitic acid for determination of creatine
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 41, Issue 6, June 2006, Pages 1371–1377
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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Any Questions? feel free to contact us