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Immobilization and characterization of laccase from Chinese Rhus vernicifera on modified chitosan

Paper ID Volume ID Publish Year Pages File Format Full-Text
36350 45129 2006 5 PDF Available
Title
Immobilization and characterization of laccase from Chinese Rhus vernicifera on modified chitosan
Abstract

Rhus laccase (RL) was immobilized on water-soluble chitosan (WSCT) and chitosan microsphere (CTMS) by various methods, and their properties were compared with transitional metal (Fe3+)-immobilized laccase in chelation. The immobilization yield (IY) on WSCT was not significantly affected by the methods used; the recovery activity of the enzyme had the relation: FeCl3-RL > RL-WSCT > RL-CTMS. The effects exist between the special dimension of the supports and the IY. After immobilization, the suitable pH and temperature of the enzyme were significantly improved. After three months at 4 °C, the losses of activity were 10% FeCl3-RL) 10% (RL-WSCT) and 15% (RL-CTMS), respectively; the activity remained 80% for RL-WSCT and 85% for FeCl3-RL after use 15 times.

Keywords
Rhus vernicifera; Rhus laccase; Chitosan; Immobilization; Enzyme activity; Oxidase
First Page Preview
Immobilization and characterization of laccase from Chinese Rhus vernicifera on modified chitosan
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 41, Issue 6, June 2006, Pages 1378–1382
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering