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Kinetics of cell growth and caffeine utilization by Pseudomonas sp. GSC 1182

Paper ID Volume ID Publish Year Pages File Format Full-Text
36355 45129 2006 5 PDF Available
Title
Kinetics of cell growth and caffeine utilization by Pseudomonas sp. GSC 1182
Abstract

Caffeine in coffee pulp prevents the utilization of nutrition rich coffee pulp effectively. Microbial methods of caffeine degradation can help in detoxifying the pulp and it prove as a better alternative to the conventional methods of environmental unfriendly solvent extraction process of decaffeination. Previously Pseudomonas sp. GSC 1182 was isolated from the soil samples of coffee cultivation area to degrade caffeine. In this report, the cell growth kinetics of Pseudomonas sp. GSC 1182 at various concentration of caffeine ranging from 0.05 to 20 g/l was studied. The kinetic data showed substrate inhibition kinetics and maximum growth rate was obtained when the isolate was grown in medium containing 2.5 g/l of initial concentration of caffeine. The results also showed that the isolate was able to grow at high concentrations of caffeine (10 g/l). Different substrate inhibition models were fitted to the kinetic data and found the Luong model was best. The model predicted kinetic parameters were in agreement with the experimental findings. The kinetic parameters were: Sm = 20.2 g/l; KS = 0.59 g/l; μm = 0.24 h−1 and n = 2. This is the first detailed report on the cell growth kinetics of microorganism using caffeine as the substrate.

Keywords
Caffeine degradation; Cell growth; Substrate inhibition kinetics; Luong model; Biomass yield; Pseudomonas sp
First Page Preview
Kinetics of cell growth and caffeine utilization by Pseudomonas sp. GSC 1182
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 41, Issue 6, June 2006, Pages 1417–1421
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering