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Enhancement of eleutherosides production in embryogenic cultures of Eleutherococcus sessiliflorus in response to sucrose-induced osmotic stress

Paper ID Volume ID Publish Year Pages File Format Full-Text
36367 45131 2006 7 PDF Available
Title
Enhancement of eleutherosides production in embryogenic cultures of Eleutherococcus sessiliflorus in response to sucrose-induced osmotic stress
Abstract

The effect of initial sucrose concentration was investigated in embryogenic cell cultures of Eleutherococcus sessiliflorus in bioreactors for production of eleutherosides, phenol, flavonoids and polysaccharide. The final dry cell weight was increased from 6.16 to 23.33 g l−1 with an increase of initial sucrose concentration from 10 to 70 g l−1, but an even higher sucrose concentration of 90 g l−1 seemed to repress the cell growth. Furthermore, it was found that a high sugar level was favorable to the synthesis of eleutherosides, phenol and flavonoids, which may be due to higher osmotic pressure. A marked increase in malondialdehyde (MDA) content in germinating embryos was observed in high sucrose treatment. MDA, a decomposition product of polyunsaturated fatty acids hydroperoxides, has been utilized very often as a suitable biomarker for lipid peroxidation, which is an effect of osmotic stress. This hypothesis is also confirmed by the higher lipoxygenase (LOX) activity under this treatment. The much higher antioxidant enzyme activities in the culture fed with higher initial sucrose suggest that osmotic stress arising from a sudden increase in medium osmotic pressure. A hypothesis of osmotic stress and eleutheroside accumulation was confirmed from these results.

Keywords
Antioxidant enzymes; Eleutherosides; Flavonoids; Osmotic stress; Phenols; Somatic embryo
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Enhancement of eleutherosides production in embryogenic cultures of Eleutherococcus sessiliflorus in response to sucrose-induced osmotic stress
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 41, Issue 3, March 2006, Pages 512–518
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
Online Support
Any Questions? feel free to contact us