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Medium optimization by orthogonal array designs for urease production by Aspergillus niger PTCC5011

Paper ID Volume ID Publish Year Pages File Format Full-Text
36371 45131 2006 5 PDF Available
Title
Medium optimization by orthogonal array designs for urease production by Aspergillus niger PTCC5011
Abstract

This paper describes medium optimization for urease production by Aspergillus niger PTCC5011 by one-factor-at-a-time and orthogonal array design methods. The one-factor-at-a-time method was used to study the effects of carbon and nitrogen sources on urease production. Among various carbon and nitrogen sources used, sucrose and yeast extract were the most suitable for urease production, respectively. Subsequently, the concentration of sucrose, yeast extract and mineral sources were optimized using the orthogonal array method in two stages. The effects of nutritional components for urease production by A. niger PTCC5011 in the first and second stages were in order of urea > NiSO4 > sucrose > KH2PO4 > K2HPO4 > CaCl2 > yeast extract > MgSO4 and yeast extract > sucrose > K2HPO4 > KH2PO4 > urea > CaCl2 > NiSO4, respectively. The optimal concentrations of nutritional components for improved urease production were determined as 20 g l−1 sucrose, 0.85 g l−1 urea, 3.4 g l−1 yeast extract, 0.03 g l−1 NiSO4·6H2O, 0.5 g l−1 MgSO4·7H2O, 0.04 g l−1 CaCl2, 5.5 g l−1 KH2PO4 and 0.35 g l−1 K2HPO4. These results showed that urea, NiSO4, yeast extract and sucrose had significant effect on urease production by A. niger PTCC5011. Tween 80 and MgSO4 had negligible effect on urease production by this strain. The subsequent confirmation experiments determined the validity of the models. Maximum urease activity in optimized media by one-factor-at-a-time and orthogonal array methods were about 1.41 and 2.74 times greater than with the basal medium, respectively.

Keywords
Aspergillus niger PTCC5011; Optimization; Orthogonal array; Production; Urease
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Medium optimization by orthogonal array designs for urease production by Aspergillus niger PTCC5011
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 41, Issue 3, March 2006, Pages 547–551
Authors
, , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us