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Enhanced production of laccase using a new isolate of white rot fungus WR-1

Paper ID Volume ID Publish Year Pages File Format Full-Text
36378 45131 2006 8 PDF Available
Title
Enhanced production of laccase using a new isolate of white rot fungus WR-1
Abstract

A new strain of white rot fungus WR-1 isolated from tree bark was found to produce high activities of laccase during fermentation. A simple medium containing glucose gave laccase activity of 124 U/ml using WR-1. The fermentation medium was optimized using a combination of one factor at a time and orthogonal array methods. Optimized medium containing 2% starch gave laccase activity of 288 U/ml. These values are very high as compared to most of the reported strains. Hence, the new isolate was further explored for enhancement in laccase production. Laccase production was enhanced considerably by incorporation of copper in the medium. Addition of 1 mM copper gave laccase activity of 410 U/ml in optimized medium. Different aromatic inducers such as 2,5-xylidine, ferulic acid, p-anisidine, etc. were used in optimized medium to further enhance laccase activity. All the inducers stimulated laccase production. The highest laccase activity of 692 U/ml was achieved using 0.8 mM 2,5-xylidine with optimized medium. Thus, the new isolate WR-1 is an efficient and excellent producer of laccase.

Keywords
White rot fungi; Laccase; Orthogonal array; Media optimization; 2,5-Xylidine; Fermentation
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Enhanced production of laccase using a new isolate of white rot fungus WR-1
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 41, Issue 3, March 2006, Pages 581–588
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us