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Production and characterisation of alginate microparticles incorporating Aeromonas hydrophila designed for fish oral vaccination

Paper ID Volume ID Publish Year Pages File Format Full-Text
36386 45131 2006 6 PDF Available
Title
Production and characterisation of alginate microparticles incorporating Aeromonas hydrophila designed for fish oral vaccination
Abstract

Oral vaccination is a relevant alternative for fish immunisation in intensive culture. However, its effectiveness is limited by possible vaccine degradation in the fish digestive system. The purpose of this work was to obtain stable biocompatible alginate microparticles entrapping inactive Aeromonas hydrophila cells for fish oral immunisation. The particles were prepared through an emulsion-based methodology, employing different vegetable oils containing the surfactant Span 80, varying the ratio between aqueous alginate and oil phases, the stirring rate and the initial concentration of A. hydrophila. Microparticles stable under gastrointestinal conditions presenting mean diameters lower than 50 μm were obtained with 1:9 (v/v) of aqueous phase containing 3.5% alginate (w/v) in relation to the oil phase (corn oil with 0.2% v/v Span 80) under stirring at 2000 rpm, with bacterium encapsulation efficiencies up to 100%.

Keywords
Microparticles; Alginate; Fish vaccine; Encapsulation; Emulsion; Aeromonas hydrophila
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Production and characterisation of alginate microparticles incorporating Aeromonas hydrophila designed for fish oral vaccination
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 41, Issue 3, March 2006, Pages 638–643
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us