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Optimization of a growth medium using a statistical approach for the production of an alkaline protease from a newly isolated Bacillus sp. L21 ☆

Paper ID Volume ID Publish Year Pages File Format Full-Text
36389 45131 2006 7 PDF Available
Title
Optimization of a growth medium using a statistical approach for the production of an alkaline protease from a newly isolated Bacillus sp. L21 ☆
Abstract

Bacillus sp. L21 was isolated from the by-products of a leather factory (located in Izmir, Turkey) working under extreme alkaline conditions. Its phenotypic and genotypic identifications were completed, and determined as a potential alkaline protease producer. After screening various elements, carbon and nitrogen sources, soybean meal, maltose50, tween80 and the initial pH conditions were chosen as main factors to be used in the experimental design and response surface methodology (RSM) for the optimization of a low cost enzyme producing media for potential use on an industrial scale. The optimized values obtained by the statistical analysis showed that soybean meal at 3.0 g/l, maltose50 between the ranges of 30 and 40 g/l, tween80 at 0.35 g/l and an initial pH of 8.0 gives maximum protease activity.

Keywords
Media optimization; Response surface method; Alkaline protease; Bacillus sp.; Low cost media
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Optimization of a growth medium using a statistical approach for the production of an alkaline protease from a newly isolated Bacillus sp. L21 ☆
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 41, Issue 3, March 2006, Pages 659–665
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us