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Modeling of the partitioning of membrane protein and phase equilibria for Triton X-100–salt aqueous two-phase systems using a modified generalized multicomponent osmotic virial equation

Paper ID Volume ID Publish Year Pages File Format Full-Text
36393 45131 2006 8 PDF Available
Title
Modeling of the partitioning of membrane protein and phase equilibria for Triton X-100–salt aqueous two-phase systems using a modified generalized multicomponent osmotic virial equation
Abstract

The partitioning of membrane protein and liquid–liquid equilibria for Triton X-100–salt–water aqueous two-phase systems were measured at 293.15 K. A generalized model was proposed to predict both partitioning of membrane protein and phase behavior of the surfactant aqueous two-phase systems. By using part of liquid–liquid equilibria (LLE) data, Triton X-100–Na2SO4 and Triton X-100–Na2CO3 aqueous two-phase systems were successfully predicted. The results showed that most of the average absolute deviations were less than 1.0% (w/w). The partition coefficient of membrane protein in Triton X-100–Na2SO4 and Triton X-100–Na2CO3 aqueous two-phase systems were also successfully predicted. The results showed that most of the average absolute deviations of the two systems were less than 5% and 8%, respectively. The model could give good representation of partitioning of membrane protein and the phase diagrams.

Keywords
Aqueous two-phase system (ATPS); Surfactant; Partition coefficient; Phase equilibria; Membrane protein
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Modeling of the partitioning of membrane protein and phase equilibria for Triton X-100–salt aqueous two-phase systems using a modified generalized multicomponent osmotic virial equation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 41, Issue 3, March 2006, Pages 689–696
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us