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A modelling study on milk lactose hydrolysis and β-galactosidase stability under sonication

Paper ID Volume ID Publish Year Pages File Format Full-Text
36407 45132 2006 8 PDF Available
Title
A modelling study on milk lactose hydrolysis and β-galactosidase stability under sonication
Abstract

In the present study, it was aimed to investigate the effect of ultrasonication as an alternative method on the kinetics of lactose hydrolysis process. The effects of the duty cycle and acoustic power of sonifier and enzyme concentration on hydrolysis of lactose and as well as on enzyme stability were studied. The lactose hydrolysis reactions were carried out using a commercial β-galactosidase produced from Kluyveromyces marxianus. Ultrasonic treatment resulted by the lactose hydrolysis degree of 90% and the residual enzyme activity of 75% at the optimum operational conditions; acoustic power of 20 W, duty cycle rate of 10% and enzyme concentration of 1 mL/L. These results show that the sonication was beneficial for lactose hydrolysis in milk. Mathematical models were also derived for residual lactose concentration and residual enzyme activity depending on the operating conditions. The predicted models have been confirmed with the experimental results.

Keywords
Lactose hydrolysis; Milk; β-Galactosidase; Sonifier; Acoustic power; Duty cycle; Enzyme concentration; Modelling
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A modelling study on milk lactose hydrolysis and β-galactosidase stability under sonication
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 41, Issue 7, July 2006, Pages 1493–1500
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us