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Production of a surfactant- and solvent-stable alkaliphilic protease by bioconversion of shrimp shell wastes fermented by Bacillus subtilis TKU007

Paper ID Volume ID Publish Year Pages File Format Full-Text
36414 45132 2006 8 PDF Available
Title
Production of a surfactant- and solvent-stable alkaliphilic protease by bioconversion of shrimp shell wastes fermented by Bacillus subtilis TKU007
Abstract

An extracellular serine protease with novel surfactant- and solvent-stable alkaliphilic properties was purified from the culture supernatant of Bacillus subtilis TKU007 with shrimp shell wastes as the sole carbon/nitrogen source. The TKU007 protease showed suppressing effect on the chitosanase, which appeared at the 1st day. The molecular mass of TKU007 protease determined by sodium dodecyl sulfate-polyacryamide gel electrophoresis (SDS-PAGE) and gel filtration was approximately 28 kDa and 30 kDa, respectively. The optimum pH, optimum temperature, pH stability, thermal stability, Km, and Vmax of TKU007 protease was 7–11, 50 °C, pH 5–11, 50 °C, 0.13 mg/mL, and 0.86 U/mL, respectively. More than 80% of its original activity was retained even after preincubation for 10 days at 25 °C in the presence of 25% tested organic solvents. Additionally, the TKU007 protease retained 100%, 100%, 50%, and 65% of its original activity in the presence of 2% Tween 20, 2% Tween 40, 2% Triton X-100, or 0.5 mM SDS, respectively. In conclusion, the novelties of the TKU007 protease include its high stability to the solvents, surfactants, and alkali. These unique properties make it an ideal choice for application in detergent formulations and enzymatic peptide synthesis.

Keywords
Bacillus subtilis; Alkaliphilic protease; Surfactant stable; Solvent stable; Shrimp shell wastes
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Production of a surfactant- and solvent-stable alkaliphilic protease by bioconversion of shrimp shell wastes fermented by Bacillus subtilis TKU007
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 41, Issue 7, July 2006, Pages 1545–1552
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us