fulltext.study @t Gmail

Optimization of cell growth and poly(ɛ-lysine) production in batch and fed-batch cultures by Streptomyces albulus IFO 14147

Paper ID Volume ID Publish Year Pages File Format Full-Text
36428 45132 2006 6 PDF Available
Title
Optimization of cell growth and poly(ɛ-lysine) production in batch and fed-batch cultures by Streptomyces albulus IFO 14147
Abstract

Streptomyces albulus IFO 14147 was cultivated in a stirred tank reactor; the pH and nutrient effects were evaluated in batch and fed-batch fermentation, particularly the control of pH and glucose feeding for enhancing the cell growth and ɛ-PL production were demonstrated. It was concluded that high cell growth was obtained and the cells remained stable at pH above 4.5 in the broth, while high ɛ-PL accumulation was achieved at lower pH; the high rate of cell growth at high pH was accompanied by a high rate of glucose consumption. To enhance the ɛ-PL production, a two-stage pH control and glucose feeding strategy was employed. At first sage the pH was maintained at its initial 6.8 for 48 h for achieving high cell density; the second stage aimed at increasing the ɛ-PL accumulation by maintaining pH at 4.0. In addition, pulsed feeding of glucose was carried out when it was depleted from the culture medium. By using this control and feeding strategy, the increase of ɛ-PL accumulation in 10-days cultivation is 258%. We have demonstrated that pH control and substrate feeding strategy is a feasible method for enhancing ɛ-PL production by S. albulus IFO 14147.

Keywords
Poly-ɛ-lysine; Streptomyces albulus IFO 14147; Fed-batch fermentation; pH control; Batch culture; Stirred tank reactor
First Page Preview
Optimization of cell growth and poly(ɛ-lysine) production in batch and fed-batch cultures by Streptomyces albulus IFO 14147
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 41, Issue 7, July 2006, Pages 1644–1649
Authors
, ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us