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Continuous production of l-phenylalanine from phenylpyruvic acid and l-aspartic acid by immobilized recombinant Escherichia coli SW0209-52

Paper ID Volume ID Publish Year Pages File Format Full-Text
36432 45132 2006 4 PDF Available
Title
Continuous production of l-phenylalanine from phenylpyruvic acid and l-aspartic acid by immobilized recombinant Escherichia coli SW0209-52
Abstract

A recombinant strain Escherichia coli SW0209-52 was immobilized with κ-carrageenan for the production of l-phenylalanine via phenylpyruvic acid (PPA) and l-aspartic acid (l-Asp). To enhance operational stability, the immobilized cells were treated with 0.05% (w/v) glutaraldehyde and the relative activity was 122.8% compared with that without treatment. The conversion yield could stay above 88% in 10 batch cycles, indicating the stability of the immobilized cells. Continuous l-phenylalanine production with PBR was studied under different feeding PPA concentrations (15–40 g/L) and residence time (2.2–10 h). Considering both conversion yield and volumetric productivity, studies on the operational stability of the reactor were conducted under the condition of 30 g/L PPA feeding concentration and 10 h residence time. The system could be operated for 40 days without significant decline and the average productivity was 1.85 g/L/h and the conversion yield was above 85% throughout the 40 days.

Keywords
l-Phenylalanine; Phenylpyruvic acid; Glutaraldehyde; Escherichia coli; Immobilization; Packed bed reactor
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Continuous production of l-phenylalanine from phenylpyruvic acid and l-aspartic acid by immobilized recombinant Escherichia coli SW0209-52
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 41, Issue 7, July 2006, Pages 1669–1672
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us