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Production of inulinase from Kluyveromyces marxianus YS-1 using root extract of Asparagus racemosus

Paper ID Volume ID Publish Year Pages File Format Full-Text
36439 45132 2006 5 PDF Available
Title
Production of inulinase from Kluyveromyces marxianus YS-1 using root extract of Asparagus racemosus
Abstract

Roots of Asparagus racemosus were used as a source of inulin for the production of inulinase from Kluyveromyces marxianus YS-1. Root extract prepared at 10 kg/cm2 for 10 min showed the maximum production of inulinase. Various parameters of fermentation have been examined in order to improve overall enzyme yield. Inulinase yield of 40.5 IU/mL in an optimized medium containing inulin (3.5%), beef extract (0.5%), MnSO4 (0.5 mM), CoCl2 (0.05 mM), SDS (0.4 mM) and pH 6.5 at 30 °C under agitation (150 rpm) for 60 h has been obtained at shake flask level. After optimization the enzyme production has been increased 6.1 times at flask level. In a bioreactor (working volume 1 L) fermentation medium containing 4% inulin has shown maximum inulinase production (47.3 IU/mL) under optimized parameters of 200 rpm, 0.75 vvm aeration at 30 °C after 60 h.

Keywords
Kluyveromyces marxianus YS-1; Asparagus racemosus; Root extract; Inulinase; Production; Optimization
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Production of inulinase from Kluyveromyces marxianus YS-1 using root extract of Asparagus racemosus
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 41, Issue 7, July 2006, Pages 1703–1707
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us