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α-Amylase from B. amyloliquefaciens: purification, characterization, raw starch degradation and expression in E. coli

Paper ID Volume ID Publish Year Pages File Format Full-Text
36448 45133 2005 8 PDF Available
Title
α-Amylase from B. amyloliquefaciens: purification, characterization, raw starch degradation and expression in E. coli
Abstract

α-Amylases of B. amyloliquefaciens and a mutant strain were purified through a series of four steps and the purity of enzymes was checked by electrophoresis. Differences were found among the enzymes with respect to pH, temperature, stability, Km and Vmax and binding of Ca. The degradation ability of wild type α-amylase on starch granules from various botanical sources (potato, sweet potato, corn, wheat and rice) were examined by scanning electron microscopy and by kinetic assays. Differences in enzyme activity on raw granules were noted. The gene coding mature enzyme was amplified by PCR and expressed in E. coli under trc and T7 promoter.

Keywords
α-Amylase; B. amyloliquefaciens; Purification; Characterization; Raw starch degradation; Expression
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α-Amylase from B. amyloliquefaciens: purification, characterization, raw starch degradation and expression in E. coli
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 40, Issue 8, July 2005, Pages 2629–2636
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
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