fulltext.study @t Gmail

Immobilization and stabilization of pullulanase from Klebsiella pneumoniae by a multipoint attachment method using activated agar gel supports

Paper ID Volume ID Publish Year Pages File Format Full-Text
36449 45133 2005 6 PDF Available
Title
Immobilization and stabilization of pullulanase from Klebsiella pneumoniae by a multipoint attachment method using activated agar gel supports
Abstract

The immobilization and stabilization of pullulanase from Klebsiella pneumoniae were investigated. Pullulanases immobilized on activated agar gel (glyoxyl-agar gel) via multipoint attachment between amino groups of pullulanase and aldehyde groups on the gel surface were significantly stable compared with free pullulanase and pullulanase adsorbed on chitosan beads. The immobilization yield, thermal stability and activity of immobilized pullulanase greatly depended on the surface density of aldehyde groups on the support gel. The optimum temperature and pH of immobilized pullulanases prepared by the multipoint attachment method using activated agar gels were 50 °C and 6.0, respectively. These values were the same as those of free pullulanase. Pullulanase immobilized on agar gel activated with 4.8 M glycidol retained 60% of its initial activity after a 250-h incubation at 45 °C, while free pullulanase completely lost its activity after a 72-h incubation at the same temperature.

Keywords
Immobilized enzyme; Pullulanase; Thermal stability; Multipoint attachment; Activated agar gel; Surface aldehyde density
First Page Preview
Immobilization and stabilization of pullulanase from Klebsiella pneumoniae by a multipoint attachment method using activated agar gel supports
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 40, Issue 8, July 2005, Pages 2637–2642
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us