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Comparative evaluation of neutral protease production by Aspergillus oryzae in submerged and solid-state fermentation

Paper ID Volume ID Publish Year Pages File Format Full-Text
36457 45133 2005 6 PDF Available
Title
Comparative evaluation of neutral protease production by Aspergillus oryzae in submerged and solid-state fermentation
Abstract

A comparative study was carried out on the production of neutral protease using agro-industrial residues as substrate in solid-state fermentation (SSF) and submerged fermentation (SmF). Seven fungal cultures, viz. three strains of Aspergillus oryzae and four strains belonging to Penicillium sp, P. funiculosum, P. funiculosum, P. pinophilum, P. aculeatum were evaluated using a plate assay for enzyme production, which showed a strain of A. oryzae NRRL 1808 as the most useful culture. Several agro-industrial residues (wheat bran, rice husk, rice bran, spent brewing grain, coconut oil cake, palm kernel cake, sesame oil cake, jackfruit seed powder and olive oil cake) were screened for neutral protease production in SmF and SSF. In both systems, wheat bran was the best substrate. Best results in SSF were obtained in a medium having an initial moisture content of 43.6%, when inoculated with 1 ml of spore suspension (8 × 108 spores) and incubated at 30 °C for 72 h (31.2 U enzyme per gram of fermented substrate – U/gds). SmF medium (pH 7.5) containing 2% (w/v) wheat bran, when inoculated with 3 ml of spore suspension and incubated at 30 °C and 180 rpm for 72 h gave maximum enzyme yield of 8.7 U/gds. A comparative evaluation of protease yield by the two fermentation systems showed 3.5-fold more enzyme production in SSF, clearly demonstrating the superiority of SSF over SmF.

Keywords
Neutral protease; Submerged fermentation; Solid-state fermentation; Aspergillus oryzae; Agro-industrial residues
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Comparative evaluation of neutral protease production by Aspergillus oryzae in submerged and solid-state fermentation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 40, Issue 8, July 2005, Pages 2689–2694
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us