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Immobilization of Streptomyces olivaceoviridis E-86 xylanase on Eudragit S-100 for xylo-oligosaccharide production

Paper ID Volume ID Publish Year Pages File Format Full-Text
36459 45133 2005 8 PDF Available
Title
Immobilization of Streptomyces olivaceoviridis E-86 xylanase on Eudragit S-100 for xylo-oligosaccharide production
Abstract

A 47 kDa xylanase (FXYN) from Streptomyces olivaceoviridis E-86 was immobilized on Eudragit S-100 and an immobilization efficiency of 90% was obtained. The optimum pH of the immobilized xylanase shifted from 5.8 to 6.3 while its optimum temperature moved from 60 to 65 °C. The immobilized enzyme also showed a higher thermal stability than the free xylanase. Only a marginal increase in the Km values of free enzyme was observed upon immobilization. However, Vmax values of the immobilized enzyme were 82–161% higher than those of the free xylanase. The immobilized xylanase was also evaluated for its application in hydrolyzing the corncob powder pre-treated with 2% NaOH solution for xylo-oligosaccharide production. The final extent of xylan hydrolysis using pre-treated corncob powder was 84% for the immobilized enzyme after 24 h of incubation at 55 °C. The immobilized xylanase retained 81% of its initial hydrolysis activity even after being recycled four times. Therefore, the immobilized xylanase is suitable to produce xylo-oligosaccahrides from corncob powder pre-treated with dilute alkaline solution.

Keywords
Eudragit S-100; Immobilization; Streptomyces olivaceoviridis; Xylanase; Xylo-oligosaccharide
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Immobilization of Streptomyces olivaceoviridis E-86 xylanase on Eudragit S-100 for xylo-oligosaccharide production
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 40, Issue 8, July 2005, Pages 2707–2714
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us