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Optimization of nutritional conditions for nattokinase production by Bacillus natto NLSSE using statistical experimental methods

Paper ID Volume ID Publish Year Pages File Format Full-Text
36466 45133 2005 6 PDF Available
Title
Optimization of nutritional conditions for nattokinase production by Bacillus natto NLSSE using statistical experimental methods
Abstract

Nattokinase is a potent fibrinolytic enzyme. The composition of the medium for the production of nattokinase was optimized with a stepwise strategy. Effects of various nitrogen and carbon sources were investigated. Fractional factorial design (FFD) was applied to elucidate the key ingredients in the media and the results indicated that the soy peptone, yeast extract and calcium chloride have a significant effect on nattokinase production. Central composite design (CCD) was employed to search for the optimal concentration of the three key components and the experimental results were fitted with a second-order polynomial model at 98% level (P < 0.02). Canonical analysis results showed that the response surface had a maximal point. According to the model, the predicted maximal nattokinase volumetric concentration was about 1273.8 units/ml and the corresponding concentration of soy peptone, calcium chloride and yeast extract were around 8.28, 0.64 and 0.74 g/l, respectively. The trial checking the developed medium showed a high nattokinase activity of 1300 ± 60 units/ml.

Keywords
Nattokinase; Bacillus natto; Medium optimization; Statistical experiment methods (SEM)
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Optimization of nutritional conditions for nattokinase production by Bacillus natto NLSSE using statistical experimental methods
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 40, Issue 8, July 2005, Pages 2757–2762
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us