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Use of a protease-modified-alumina complex to design a continuous stirred tank reactor for producing bioactive hydrolysates

Paper ID Volume ID Publish Year Pages File Format Full-Text
36477 45133 2005 8 PDF Available
Title
Use of a protease-modified-alumina complex to design a continuous stirred tank reactor for producing bioactive hydrolysates
Abstract

For continuous preparation of bioactive peptide-containing fractions, a CSTR was set up with pepsin immobilized on a new support: acidic alumina treated with 2-ethanolamine-O-phosphate (2-EAOP). Hydrolyses were performed at pH 3; 23 °C. Kinetic studies were focused on the appearance of two opioid peptides (LVV-haemorphin-7, VV-haemorphin-7), an analgesic peptide (neokyotorphin) and an antibacterial peptide (α1–23 peptide). The optimal concentration of LVVh-7 was obtained after 2 h incubation. This incubation time was chosen to be the residence time for continuous production of a hydrolysate enriched in active transient peptides. Stationary concentrations of these peptides were obtained for more than 20 h at the output of the CSTR. Respectively, 30 mg LVVh-7, 28 mg VVh-7 and 28 mg α1–23 antibacterial peptide were produced from 5 g bovine haemoglobin after 20 h of reactor working. Neokyotorphin could not be prepared by this way because of too low production.

Keywords
CSTR, continuous stirred tank reactor; 2-EAOP, 2-ethanolamine-O-phosphate; N.K., neokyotorphin; VVh-4, VV-haemorphin-4; LVVh-7, LVV-haemorphin-7; VVh-7, VV-haemorphin-7; RP-HPLC, reversed phase high pressure liquid chromatography; TNBS, trinitrobenzene su
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Use of a protease-modified-alumina complex to design a continuous stirred tank reactor for producing bioactive hydrolysates
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 40, Issue 8, July 2005, Pages 2841–2848
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us