fulltext.study @t Gmail

Detection of aflatoxigenic fungi in selected food commodities by PCR

Paper ID Volume ID Publish Year Pages File Format Full-Text
36479 45133 2005 6 PDF Available
Title
Detection of aflatoxigenic fungi in selected food commodities by PCR
Abstract

Aflatoxins are toxic secondary metabolites produced by Aspergillus flavus, Aspergillus parasiticus and Aspergillus nomius. A regulatory gene, aflR, is involved in regulation of aflatoxin biosynthesis and the sequence has been published. In this study, rapid assessment of aflatoxigenic fungi in food was accomplished using an indigenously designed primer pair for the aflatoxin regulatory gene aflR in polymerase chain reaction (PCR). Specificity was assayed in pure and mixed culture systems using DNA extracted from 28 different fungal strains as PCR template. Positive amplification was achieved only with DNA from aflatoxigenic A. flavus and A. parasiticus. The detection limit for mycelium and spores was determined as 0.05 g and ≥100 cfu, respectively. Specificity and sensitivity of PCR assay in groundnuts and maize for A. flavus was possible with as few as 100 cfu/g.

Keywords
Aflatoxins; Aspergillus flavus; Aspergillus parasiticus; PCR; aflR; Food commodities
First Page Preview
Detection of aflatoxigenic fungi in selected food commodities by PCR
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 40, Issue 8, July 2005, Pages 2859–2864
Authors
, , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us