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Hydrogen peroxide detection system consisting of chemically immobilised peroxidase and spectrometer

Paper ID Volume ID Publish Year Pages File Format Full-Text
36498 45134 2005 5 PDF Available
Title
Hydrogen peroxide detection system consisting of chemically immobilised peroxidase and spectrometer
Abstract

This paper describes a simple, fast and sensitive method for the determination of hydrogen peroxide in liquid samples. The system, consisting of a mini-reactor containing horseradish peroxidase (HRP) immobilised onto polyaniline coupled to a spectrophotometer, was able to determine hydrogen peroxide in the range from 55 to 2730 nmol L−1 using pyrogallol as the reduced substrate. The determinations were made using a flow injection system with a flow rate of 3.0 mL min−1 and optical detection at 420 nm with a glass flow cell having a 1 mm optical path length. The system has good response repeatability (R.S.D. 0.3–2.3%), while the mini-reactor can be stored and reused with up to 1500 injections without significant reduction of enzyme activity.

Keywords
Spectrophotometric biosensor; Hydrogen peroxide; Peroxidase; Polyaniline; Stability; High sensitivity
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Hydrogen peroxide detection system consisting of chemically immobilised peroxidase and spectrometer
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 40, Issue 11, November 2005, Pages 3441–3445
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us