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Optimization of the amino acid composition in glutathione fermentation

Paper ID Volume ID Publish Year Pages File Format Full-Text
36503 45134 2005 6 PDF Available
Title
Optimization of the amino acid composition in glutathione fermentation
Abstract

In this study, uniform design (UD) method was applied to obtain the optimal amino acid composition in glutathione fermentation by S. cerevisiae T65. Glutamic acid, glycine and cysteine were the three most important amino acids in the synthesis of glutathione. A uniform design experiment with three factors (glutamic acid, glycine and cysteine) at five levels was designed according to the uniform design table U15(53). An optimal amino acid composition of 10 mM glutamic acid, 18 mM glycine, and 3.35 mM cysteine was found. When applying the optimal amino acid composition to the flask culture, the glutathione content in yeast cells reached 1.836%, which was 127% higher than the control.The experiments showed that the optimal strategy of amino acid addition in flask culture comprised two steps: first, 2 mM cysteine was added after 2 h; second, the combined amino acids (10 mM glutamic acid, 18 mM glycine, and 3.35 mM cysteine) were added after 7 h.When the optimal amino acid addition strategy was applied to the batch fermentation, the glutathione yield reached 329.3 mg/L. The glutathione content was 1.67%, which was 72.2% higher than the control.

Keywords
Amino acid; Uniform design; Glutathione; Fermentation; Optimization; Saccharomyces cerevisiae
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 40, Issue 11, November 2005, Pages 3474–3479
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us