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Extracellular alkaline protease from a newly isolated haloalkaliphilic Bacillus sp.: Production and optimization

Paper ID Volume ID Publish Year Pages File Format Full-Text
36516 45134 2005 7 PDF Available
Title
Extracellular alkaline protease from a newly isolated haloalkaliphilic Bacillus sp.: Production and optimization
Abstract

A newly isolated haloalkaliphilic Bacillus sp., Ve1, produced substantial levels of extracellular alkaline protease. Enzyme production corresponded with growth and reached a maximum level (410 U/ml) during the early stationary phase along with a brick red pigmentation. Protease production was maximum (397 U/ml) in gelatin broth. While growth and protease production was optimum at 10% (w/v) NaCl, only marginal growth without enzyme production was evident in the absences of salt. Ve1 could grow and produce protease at pH 7–9, the optimum being at 8 and 9, respectively. Among the organic nitrogen sources used, growth was best supported by a combination of peptone and yeast extract, while the optimum protease production was with casamino acid followed by gelatin. Enzyme production was highly reduced in soya peptone, trader's protein and tryptone. Inorganic nitrogen sources proved to be less favourable. Strong catabolic repression on protease production was observed with glucose and ammonia. The study assumes significance in the light of increasing emphasis on biocatalysis under more than one extreme condition.

Keywords
Alkaline protease; Haloalkaliphiles; Catabolite repression; Extremophiles; Bacillus sp.; Protease optimization
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Extracellular alkaline protease from a newly isolated haloalkaliphilic Bacillus sp.: Production and optimization
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 40, Issue 11, November 2005, Pages 3569–3575
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us